An in-vivo herb-drug interaction study of Tinospora cordifolia extract on the pharmacokinetics of gliclazide in normal Wistar rats

Background and Aims: Tinospora cordifolia (Willd.) Miers (TC), from the Menispermaceae family, is a well-known traditional herb used to treat diabetes mellitus variety of other ailments, according Indian literature. Herbal treatments are commonly as alternatives medicines. Anti-diabetic herbal medicines containing TC available in market. has been identified powerful CYP2C9 inhibitor, boosting risk herb-drug interactions (HDI) when with medications metabolized via CYP pathway. The present study evaluated pharmacokinetic HDI extract Gliclazide (GL) after oral co-administration Wistar rats using vivo studies. Methods: A simple, sensitive & accurate RP-HPLC/PDA method was developed validated. Chromatographic separations were performed on C18 analytical column mobile phase acetonitrile 40 mM ammonium acetate buffer (55:45 v/v) pH 3.0 flow rate gradient programming. drug concentrations plasma measured RP-HPLC (100 mg/kg) GL (8.3 rats. Results: (PK) interaction studies showed that bioavailability had significantly increased, significant influence Cmax, AUC0-48, suppression volume distribution (Vd), no change Tmax. Conclusion: results obtained this proposed there potentially PK administered together. This knowledge potential could be beneficial healthcare providers diabetic patients medication. Further research needed anticipate pharmacokinetics humans.